Human umbilical cord mesenchymal stem cells attenuate experimental pulmonary arterial hypertension

Abstract Background Pulmonary arterial hypertension (PAH) is characterized by the remodeling of pulmonary arteries, with an increased pressure and right ventricle (RV) overload. Until now, there still a lack effective treatment applied. Experimental research has reported beneficial effects mesenchymal stem cells (MSCs) therapy in PAH. However, are few studies on human umbilical cord (hUC-MSCs) transplantation Purpose The purpose present study was to observe protective effect hUC-MSCs against experimental PAH colonization lung tissue. Methods Sprague Dawley rats were randomly divided into three groups: control group, monocrotaline (MCT)-induced cell group (PAH+ hUC-MSCs). Rats induced one-time intraperitoneal injection 60mg/kg (PAH PAH+hUC-MSCs groups) at beginning experiment, while received saline (SAL) instead. Huc-Mscs treated tail vein twice 1 2 weeks later (PAH+huc-MSC dose: 3x106/each time), Ctr groups given same volume SAL vein. On day 28, Right ventricular systolic pressures (RVSP) measured cardiac catheterization. WT% WA% arterioles evaluated ipp6.0 Image Analysis Software. hypertrophy index (RVHI) weighing method. tissue detected immunohistochemistry. Results RVHI RVSP MCT-induced significantly higher than those (P<0.05). Compared RVHI, RVSP, WT%, lower Immunohistochemical examination showed that nuclear staining brown, no observed group. Conclusions In PAH, can transplanted tissues injection. reduced vascular remodeling, improved hemodynamics. Funding Acknowledgement Type funding sources: Foundation. Main source(s): National Natural Science Fundation China (82060018) Yunnan (202101AS070043, 202102AA31003-7).